Hybridoma and Cell Culture Shared Resource Facility
Harry D. Bear, M.D., Ph.D.
Resource director
(804) 828-9325
hdbear@vcu.edu
Teri L. Russell
Resource manager
(804) 828-9221
Services
- Custom monoclonal antibody production.
- Mycoplasma screening.
- Cell culture – including large scale production.
- Isotyping of antibodies.
- Expansion and cryopreservation of cell lines.
- LN2 cell storage.
- Supply of LN2 to investigators.
- Ascites production.
- Teach lab personnel cell culture/hybridoma techniques.
- Clinical trials – participation at various stages of protocol.
Resources and environment
- The Hybridoma and Cell Culture Laboratory is located in room 1-135 in the Massey Cancer Center building.
- The laboratory contains a cell culture room housing a 4-foot biohazard laminar flow hood, water-jacketed CO2 incubator, phase contrast microscope. A larger general laboratory is equipped with a 6-foot biohazard laminar flow hood, standard binocular microscope, pH meter, balance, refrigerator, centrifuge, -20C and -80C freezer and a large CO2 incubator. The room also houses a standard autoclave, a Millipore reagent-grade water purification system, a 165 liter liquid nitrogen storage tank and four liquid nitrogen freezers for cell storage. The laboratory is equipped with a PC, Costar Transplate system and an automatic ELISA microplate reader. Finally, a virus-free animal housing facility for mice and rats is located in the same building and is equipped with laminar flow cage shelves.
Service access and charges
Monoclonal antibody production |
Cost |
|---|---|
1. Immunization (three mice) |
See #4 cost |
2. ELISA screening for antibody production and titration of antigen |
See #4 cost |
3. Fusion |
See #4 cost |
4. Hybridoma maintenance – two weeks |
$1,000 (Items 1-4) |
5. ELISA screening for antibody secreting hybridoma |
$35/hour |
6. Cloning by limiting dilution |
$35/hour |
7. Rescreening by ELISA |
$35/hour |
8. Subcloning by limiting dilution |
$35/hour |
9. Rescreening by ELISA |
$35/hour |
10. Expansion of cloned hybridoma |
$35/hour |
11. Cryopreservation of cloned hybridoma |
$35/hour |
12. Storage of hybridoma cell lines (one cane=five vials) |
$1/cane/month |
|
|
Mycoplasma assay |
$35/sample |
Ascites production |
$35/hour plus cost of mice |
Expansion and cryopreservation of cell lines |
$35/hour |
Cell culture/large scale production |
$35/hour plus supply cost(s) |
Supply of LN2 |
$0.50/inch |
Note – Subsidized fee structure is for Massey Cancer Center members and research faculty at VCU only. Requests for service from outside institutions or organizations are billed at actual cost (~2X).
