Virginia Commonwealth University

VCU Massey Cancer Center

Services and rates

Services

The Biological Macromolecule Shared Resource Core provides the services listed below. If you are a Massey member, the cost of these services is partially subsidized as a member benefit.

  • Plasmid preps
  • Transformations
  • Insert purifications
  • siRNA’s through Invitrogen and Qiagen
  • Genomic DNA isolation
  • Virus construction, purification and Tttering services:
    • Adenovirus
    • Retrovirus
    • Lentivirus
    • Mouse retrovirus
  • Protein expression
  • Protein production & purification
  • Design, construction and validation for generation of new vectors
  • Dry ice and liquid nitrogen

Rates and member subsidy

Rates are current as of 27 May, 2011, but are subject to change. Massey members pay 75 percent of these rates per the member subsidy program.

Adeno-Associated Virus (AAV)

  • Supernatant generation (10ml) and infection of cells, each $100
  • Titer, each, inquire

Adenovirus

  • Adenovirus, crude prep, each $50
  • Adenovirus titer, each $100
  • Construction of adenovirus, each $240
  • CsCl gradient purification, each $440
  • Test for replication competent adenovirus, each $200

Cell culture

  • Antibody production, each $80
  • Clone isolation, each $70
  • Transfection, each $100

Commodities

  • Dry ice, per lb $2.50
  • Nitrogen, cubic inch $0.02
  • siRNA, inquire

Insert purification

  • Each additional ug purified, each $11.00
  • First ug purified, each $27.00

Instrumentation usage

  • AlphaInnotech, per hour, inquire
  • Typhoon, per hour, inquire
  • VersaDoc 4000, per log in $2.50
  • VersaDoc 4000, usage minute $0.05

Plasmid preps

  • Miniprep, Endotoxin-Free, each $25
  • Midiprep, Endotoxin-Free, each $55
  • Maxiprep, Endotoxin-Free, each $70
  • Megaprep, Endotoxin-Free, each $130

Retrovirus/Lentivirus

  • Large dupernatant generation, 30ml, each $200
  • Supernatant generation, 10ml, each $100
  • Titer - each, inquire

Special projects - DNA

  • Gene synthesis, each, inquire
  • Genotyping/LOH analysis, each, inquire
  • Library screening, each, inquire
  • Methylation analysis, each, inquire
  • Site directed mutagenesis, each, inquire
  • Subcloning, each, inquire

Special projects - protein

  • Additional purification, large, each $200
  • Additional purification, small, each $200
  • Bacterial cell prep, large, each $275
  • Bacterial cell prep, small, each $200
  • Initial purification, large, each $500
  • Initial purification, small, each $200
  • Insect cell prep, large, each $750
  • Insect cell prep, small, each $250
  • Mammalian cell prep, each, inquire
  • Solubilization, each $100

Tissue/Cell culture miniprep

  • Genomic DNA purification, each $10
  • RNA purification, each $10
  • microRNA purification, each $10

Transformation

  • Transformation, each $27

Technical consultation

  • Consultation, hour $50

Massey member subsidy benefit

As of January 1, 2012, VCU Massey subsidizes 25 percent of the cost of members’ core laboratory usage for the following Massey-supported, fee-for-service shared resources:

  • Biological Macromolecule Shared Resource Core
  • Flow Cytometry Shared Resource Core
  • Lipidomics Developing Shared Resource Core
  • Structural Biology Shared Resource Core
  • Tissue and Data Acquisition and Analysis Core
  • Transgenic/Knock-out Mouse Facility
  • VCU Microscopy Facility

Note that this subsidy is available only for Massey members. To receive this member benefit, please initiate core lab services using a VCU index code associated with your name. When you are billed for core laboratory usage, the CORES system will automatically split the invoice between your index code and a Massey account established for this purpose. You will be charged 75 percent of the published rate and VCU Massey will provide a 25 percent co-pay to cover the balance.

Please direct any questions or concerns about the member subsidy benefit to Paul Fawcett, Ph.D. at pfawcett@vcu.edu or 804-833-1959.