VCU Massey Cancer Center


Suggested grant language

Investigators contemplating the inclusion of core laboratory services in a pending grant application are strongly encouraged to consult with the faculty core director in order to craft grant language tailored to a particular application, and to develop a suitable budget for the proposed services. However, as a starting place, the following language may be used as a template:

The VCU Macromolecule Core Laboratory (Director, Shirley Taylor, Ph.D.), newly established in 2010 by the consolidation of the former Molecular Biology and Virus Vector core laboratories, provides a range of services to the VCU research community on a fee-for-service basis. The focus of the core is the production of biological macromolecules for multiple endpoints. In addition to routine molecular biology support services such as DNA preparations, insert purification, and DNA transformation, value-added services include the production of research grade vector virus particles (adenovirus, adeno-associated virus, retrovirus, and lentivirus), production of targeting vectors for the production of knock-in/knock-out mice (in conjunction with the transgenic mouse core) and somatic cell lines, and other specialized or challenging custom DNA projects. With the addition in 2010 of a new Co-Director (Darrell Peterson, Ph.D., a protein biochemist), the core is now able to offer an extensive range of protein expression and purification services. Recombinant proteins are available from bacterial, yeast, and mammalian origin. The core laboratory is housed in two locations. There is a 900 sq ft general molecular biology lab on the 6th floor of Sanger Hall with a 100 sq ft tissue culture area and a 150 sq ft office. In addition, viral vectors are produced in an access-limited 900 sq ft facility equipped with a Forma double-unit CO2 incubator, a Nikon tissue culture microscope, and a Beckman ultracentrifuge. Bacterial protein production is carried out in a facility at the Virginia Biotech Research Park using a BioFlo4500 biofermentor, supported by additional equipment available in Dr. Peterson's adjacent protein purification laboratory. The Macromolecule core laboratory is supported, in part, by funding from the NIH-NCI Cancer Center Support Grant (P30 CA016059).”