Macromolecular structure is fully integrated into basic studies of disease and the development of therapies. X-ray crystallography determination methods provide the majority of the high resolution structures which constitute the database for understanding molecular function and interactions at the atomic level. X-ray crystallography supports research ranging from rational design of competitive and mechanism-based inhibitors and effectors of enzyme function to studies of the effects of disease-related mutations on in vivo function .
The X-ray crystallography arm of the Structural Biology Shared Resource provides the instrumentation and expertise to attack problems related to understanding cancer and developing therapeutic agents. The X-ray crystallography suite is located in Biotech I., and available resources include:
- Rigaku Raxis-IV++ imaging plate system (right)
- MicroMax-007 high frequency rotating anode
- Blue Max-Flux Confocal optical system, x-stream cryogenic system
- RAXIS-IV++ 2phi stage and Windows-based CrystalClear software for data acquisition and processing
Data acquisition with CrystalClear
In addition, the facility has recently acquired a CrysCam digital microscope to facilitate the monitoring of crystal generation screening and the harvesting of crystals.